Dear Dr. Finer, We have an 1.8kb upstream region of a predicted gene in...

Asked March 18, 2015, 3:52 AM EDT

Dear Dr. Finer, We have an 1.8kb upstream region of a predicted gene in asparagus which we would like to study further. One of our first questions would be to identify cis-regulatory elements; the gene is expressed at low levels. Since we are new to this and started a literature study, we came across your review article of 2013 and some databases including PLACE and CARE. I hope you can be of assistance with identifying CRE predictions and/or directions on how we should tackle this. Thanks in advance! >upstream sequence including atg GGGCCAACCCCGGACCGGGCCCGGACTGACGGGCTTTTTGGATTGGCCTAAACATATGCATTTATCTTTAAAAGTCCATGTTATTTTTATGGGCTTGTTGCAAGCAAGCATGGCGGTAAACTAATCCTGGGTTTCAACATTAGTGCTAGTAAAGCTTTGCCATCTTTTTTTTTTTTGCTTTAAAGGGCCAACCCCGGACCGGGCCCGGACTGACGGGCTTTTTGGATTGGCCTAAACATATGCATTTATCTTTAAAAGTCCATGTTATTTTTATGGGCTTGTTGCAAGCAAGCATGGCGGTAAACTAATCCTGGGTTTCAACATTAGTGCTAGTAAAGCTTTGCCATCTTTTTTTTTTTTGCTTTAAAGTAATTTTTTAGTAAACTTTTATTCTTAATAGTTCACAACAACACGAGAACCATACAAGTGAAAGAAATTTTATCCCCAAAAAATTTCAAATAACCATCTCCAATTTTCACCAAATAATCCATCACAATTATCTATATTATACGCAATTCCACTGTTTTCTCGATTTTTTAACTTTACAAGTCATTAGATTCCTAGCACACAAAATTACCATTGTTTGTGGGAGGAAAAAAGGCATATTCTACGCTTACAACCCAAGTCACACGATAATCACATTCTTCAAGCTTATGAGGAAACTTCGTCCTATTCCCCCAACTTAAATTATTACAAAAAATCAGGAATAAGGAAAAAAGAAAAAATTTCCTTCTCTAAAATTTCAACTAAGACCATTATCAACTTCTTCCAAATAATTTGTACCCATCATCAGTTTCTACCGTTCTCACTCATCGAAACATAAATTTTACAGTTTCTACAAGAATAAAATAAACAAGACTGAGTCATAGAGAGAGGAGAAGATGGCAGCGAGATCGGAGGAGAAGATCTTGTCGAACTAGTAGGGATGAGCTCAAGGTTGGTGGTGTTGGCACTGGGGGATGGGGAGAGATAGACGATGGGATTCGACGGAAAGATGAAGAGGGAGAGGAGGAGGGTTAGGAGAAAGTAGGAGAGGAGGTTGAATCGAAAGTTGAGAATGACGACGAGCTCGAGAAAGGAGTAGATGGCAGCGAGTTTGAAGGAGAAGATCTTGTCAAACAAGTTGAGCATGAGCTCAAGGTCGGTAGTGGCGAAGGGCAAGGCGACAGAGTTAGACGGCAGATGCCAAGGAGAAGATGAAGAGGGAGAGGAGGAGGCTAAGGAGAGAGTAGAAAGAGATGAGGCTGAATCACAAATGGGACGAAAGTTATGAGATCGAAGAGGGGTCTTTTCGGAATTCTCAAACGACATTTCTTGGGGTAGAGTGAAAAGAGGGCTGAATCAAAAAGATGCAATGTATTTGGGGCTAAAATACAAGAGGGAAATAATACGGTGGCTTGAGACACATCAAAGTTTAAATTTTAGGGCCTTCACACAACTTTCCCAATTTTAAATTCTTGCTCTCCTAACCAAACGCAAAACAAAAGAAAAAAAACCGAAAAAAAAAGAAGAGAAAAATTAATAACAGGAAGAGGCCGAAAGAGGCTTTTATTTTCCATTTCACTGGTGAGGGAGGAAGATTAACTGAACCTCCCCTTTATACCCTCTAGTTATTCTAGCGACACGAACTCAACTCCAGGTGACAACATTCATAGAGACGATAAACCTCATCACCTCATATGCACCTTCACAACAATTACCATCTCCTGACAGCAAACCCGCCGGTCCCATATCAGCCCTCTGCATTCGCCTCCCTTCGAGCTCCCTTCTCAAACCTGTCTTCTCCAAGTTCCAAGATTCACCTTCTTGAACAAGATTCAAGAACATG Kind regards, John Groenendijk Wageningen University The Netherlands

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