Dear Sir,I would be very much grateful to you if you kindly clarify my...
I would be very much grateful to you if you kindly clarify my confusion. I am working on diversity analysis of sugarcane (51 genotypes) using SSR markers. So far I know that band present in certain genotype is counted as the no. of allele. Suppose SSR marker (SMC 119) produced two fragments e.g. 150 bp and 180 bp. DNA fragment of 150bp found in 20 genotypes. Can I record total no. of band (20) ( produced by SMC 119) is equal to total no. of allele present (20)?
Md. Raihan Ali
Dear Raihan Ali,
Your diversity panel is polymorphic for SMC199. The two size fragments represent two alleles for SMC119 in your population of 51 genotypes. The 150bp allele is found in 39% (20 of 51) of this population.
I hope this helps.